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The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a <t>COMSTAT</t> algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).
Comstat Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a <t>COMSTAT</t> algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).
Spm8 Software, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a <t>COMSTAT</t> algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).
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Esri inc cityengine 2015.1
The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a <t>COMSTAT</t> algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).
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The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a <t>COMSTAT</t> algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).
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The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a <t>COMSTAT</t> algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).
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The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a <t>COMSTAT</t> algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).
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The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a COMSTAT algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).

Journal: Microorganisms

Article Title: Marine Bacteria Display Different Escape Mechanisms When Facing Their Protozoan Predators

doi: 10.3390/microorganisms8121982

Figure Lengend Snippet: The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4 and Shewanella sp. TC10, Polaribacter sp. TC5, and Shewanella sp. TC11 biofilms. The 24 h- and 48 h-old Persicivirga mediterranea TC4 ( I ) and Shewanella sp. TC10 ( II ), Polaribacter sp. TC5 ( III ) and Shewanella sp. TC11 ( IV ) biofilms biovolumes were monitored using CLSM after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), amoebae ( B ), and the amoeba supernatant ( C ). Pictures are representative of three biological replicates. Bar plot represents the calculated biofilm biovolume in the different conditions relative to the incubation time ( D ). The biovolumes were evaluated by a COMSTAT algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation, and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (a ≠ α).

Article Snippet: The biovolume of CLSM pictures was determined with the COMSTAT software developed in MATLAB R2015 a (Mathworks, Natick, MA, USA) [ ].

Techniques: Control, Incubation, Standard Deviation

The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4, Shewanella sp. TC10, and Shewanella sp. TC11 in multi-species biofilms. Multispecies biofilm formation including Persicivirga mediterranea TC4, Shewanella sp. TC10, and Shewanella sp. TC11 in the presence of A. castellanii and A. castellanii supernatant. The formation of 24 h- and 48 h-old biofilms was monitored using CLSM (confocal laser scanning microscopy) after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), the amoebae supernatant ( B ), and the amoebae ( C ). Bar plots represent the calculated biofilm biovolumes in the different conditions ( D ). Pictures are representative of three biological replicates. Biovolumes were evaluated by a COMSTAT algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (A≠ a ≠ α≠ γ, B ≠ b ≠ β ≠ γ and A≠B, a≠ β).

Journal: Microorganisms

Article Title: Marine Bacteria Display Different Escape Mechanisms When Facing Their Protozoan Predators

doi: 10.3390/microorganisms8121982

Figure Lengend Snippet: The presence of A. castellanii and its supernatant have an effect on Persicivirga mediterranea TC4, Shewanella sp. TC10, and Shewanella sp. TC11 in multi-species biofilms. Multispecies biofilm formation including Persicivirga mediterranea TC4, Shewanella sp. TC10, and Shewanella sp. TC11 in the presence of A. castellanii and A. castellanii supernatant. The formation of 24 h- and 48 h-old biofilms was monitored using CLSM (confocal laser scanning microscopy) after the addition for an additional 24 h of NSS (nine-salt solution) (control) ( A ), the amoebae supernatant ( B ), and the amoebae ( C ). Bar plots represent the calculated biofilm biovolumes in the different conditions ( D ). Pictures are representative of three biological replicates. Biovolumes were evaluated by a COMSTAT algorithmic method. Values are the means of three biological replicates, error bars represent standard deviation and significant differences between incubation times ( p -value < 0.05) are displayed by different letters ( a – c ) in a same alphabet (A≠ a ≠ α≠ γ, B ≠ b ≠ β ≠ γ and A≠B, a≠ β).

Article Snippet: The biovolume of CLSM pictures was determined with the COMSTAT software developed in MATLAB R2015 a (Mathworks, Natick, MA, USA) [ ].

Techniques: Confocal Laser Scanning Microscopy, Control, Standard Deviation, Incubation